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The material disruption was carried out using a vibrating-ball micromill. Then the homogenized tissue was GA induction in second leaf sheath elongation, internode incubated in the 80% acetone for 1 h at room temperature (25 C). elongation and a-amylase activity After centrifugation, the solvent was removed in a vacuum centri- fuge. ...

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Jun 19, 2003· Plant leaf material (0.1 g fresh weight) was collected in a 1.5-ml Eppendorf cup, frozen in liquid nitrogen and macerated for 1 min in a vibrating-ball micromill (MM300; Qiagen) at 25 s −1. After adding 0.4 ml of homogenization buffer [50 mM HEPES, 0.2 M sucrose, 3 mM EDTA, 0.2 mM phenylmethylsulfonyl fluoride (pH 7.5), containing 0.6% (w/v ...

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vibrating-ball micromill (Retsch MM301, Haan, Germany) at a vibration fre quency of 30 s-1. After vortexing and. incubation for 15 min at room temper ature, the extract was.

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Grinding bowl oscillations per minute. 3,000 – 3,600 at 1-3 mm amplitude. Electrical details. 100-240 V/1~, 50-60 Hz, 50 Watt. Emission sound pressure level at the workplace according to DIN EN ISO 3746 (depending on the material to be ground and mortar/grinding balls used) approx. L pAd = 68 dB, with sound absorption hood approx.L pAd = 53 dB.

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Jun 14, 2013· The tube was immediately exposed to 60 °C for 20 min and the content subsequently disrupted for 20 min using a vibrating-ball micromill (Retsch MM301, Haan, Germany) at a vibration frequency of 30 s −1.

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